KMID : 0880220170550080612
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Journal of Microbiology 2017 Volume.55 No. 8 p.612 ~ p.618
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Imipenem-resistant Gram-negative bacterial isolates carried by persons upon medical examination in Korea
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Kim So-Yeon
Shin Sang-Yop Rhee Ji-Young Ko Kwan-Soo
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Abstract
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Carbapenem-resistant Gram-negative bacteria (CR-GNB) have emerged and disseminated worldwide, become a great concern worldwide including Korea. The prevalence of fecal carriage of imipenem-resistant Gram-negative bacteria (IR-GNB) in persons in Korea was investigated. Stool samples were collected from 300 persons upon medical examination. Samples were screened for IR-GNB by using MacConkey agar with 2 ¥ìl/ml imipenem. Species were identified by 16S rRNA gene sequence analysis, and antimicrobial susceptibility was determined by the broth microdilution method. In total, 82 IR-GNB bacterial isolates were obtained from 79 (26.3%) out of 300 healthy persons. Multilocus sequence typing analysis showed very high diversity among IR P. aeruginosa, S. maltophilia, and E. cloacae isolates, and pulsed-field gel electrophoresis revealed five main pulsotypes of IR P. mirabilis. As for the presence of metallo-¥â-lactamases (MBLs), only one IMP-25-producing S. marcescens isolate was identified. Although only one carbapenemase-producing isolate was identified, the high colonization rates with IR-GNB isolates in this study is notable because carriers may be a reservoir for the dissemination of resistant pathogens within the community as well as in health care institutions.
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KEYWORD
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Imipenem resistance, fecal carriage, Proteus mirabilis, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Enterobacter cloacae
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